mRNA/ total RNA sequencing
RNA seq is a highly sensitive and accurate tool for measuring the level of gene expression across transcriptomes. With NGS technology, the expression level of mRNA can be expressed by the number of reads to obtain accurate data. It provides information on the changes caused by various disease conditions, treatments responses, and different environmental conditions. Scientist can detect not only the amount of expression between genes, but also novel transcriptome, fusion gene, alternative specification, and mutation. The expression profile and mutation information between samples provide insight into gene-enabled research and expression control, and can be applied to biomarker development, disease diagnosis, and monitoring.