Research Services 목록

Whole Genome Sequencing

Next generation sequencing service

Genome Insight provides NGS experiment and analysis services for domestic and foreign researchers. We are a leading global precision medicine company with algorithms and IT analytics that handle massive amounts of sequencing data quickly and accurately, increasing and scaling WGS capabilities to provide a platform for real-world clinical use of big data. We will provide you with differentiated experimental and analysis results produced by highly qualified researchers and bioinformatics experts at a quick TAT.

Whole genome sequencing Whole genome re-sequencing
Whole genome de novo sequencing
Epigenetics & immune profiling ChiP sequencing
HLA typing
Bulk TCR/BCR sequencing
Transcriptome sequencing mRNA sequencing total RNA sequencing
Single cell multi-omics sequencing Single cell RNA sequencing
Single cell nucleus RNA sequencing
Single cell immune profiling (TCRs, BCRs)
Single cell ATAC sequencing

Whole genome sequencing

Whole genome re-sequencing

WGS is the process of determining the entire genetic information by sequencing the whole genome and analyzing its genetic variations. Using a reference genome, you can analyze diverse variations such as single nucleotide variant (SNV), insertion & deletion, copy number variation (CNV), and structural variation (SV). Variant information obtained through WGS is being used to discover candidate genes associated with disease and drug response to pave the way for personalized precision medicine.

Whole genome de novo sequencing

De novo sequencing refers to sequencing a novel genome such as microorganisms and animals and plants where there is no reference sequence available for alignment. Genome insight offers rapid, comprehensive, accurate characterization of any species.

Sequencing platform and product

Sequencing platform	NovaSeq 6000 sequencing system, Illumina
Library prep	TruSeq PCR-free Nano, Illumina

Transcriptome sequencing

mRNA/ total RNA sequencing

RNA seq is a highly sensitive and accurate tool for measuring the level of gene expression across transcriptomes. With NGS technology, the expression level of mRNA can be expressed by the number of reads to obtain accurate data. It provides information on the changes caused by various disease conditions, treatments responses, and different environmental conditions. Scientist can detect not only the amount of expression between genes, but also novel transcriptome, fusion gene, alternative specification, and mutation. The expression profile and mutation information between samples provide insight into gene-enabled research and expression control, and can be applied to biomarker development, disease diagnosis, and monitoring.

Sequencing platform and product

Sequencing platform	NovaSeq 6000 sequencing system, Illumina
Library prep	TruSeq Stranded Total RNA, Illumina/ KAPA Hyper RNA, Roche

Single cell, multi-omics sequencing

Single cell RNA sequencing

Live tissues are made up of a variety of cell types with unique functions, and heterogeneity is observed between subpopulations of cells and between cells of different types. Because of this complexity, individual cells must be separated and analyzed to identify the characteristics of each cells. Single cell RNA sequencing (ScRNA-seq) separates a group of cells into a single cell and analyzes gene expression using NGS technology. Unlike bulk RNA sequencing which combines various cells to obtain average values, single cell RNA sequencing allows analysis of cellular heterogeneity and rare cell populations in a single cell to study unknown and complex cell processes.

Single cell nucleus RNA sequencing

Sensitive cells that are easily damaged in the separation process or difficult to separate during sample preparation are difficult to analyze. Single cell nucleus RNA sequencing is a technology that overcomes these limitations and is a method of analyzing transcriptome by separating a single nucleus. While fresh tissue samples are recommended for ScRNA-seq, single cell nucleus RNA sequencing also allows analysis from frozen samples and transcription from tissues that are difficult to separate, reducing stress and bias that may occur during single cell separation.

Single cell immune profiling (TCRs, BCRs)

Sequencing VDJs and VJ genes involved in the diversity of clonotypes at a single cell level enables immune profiling of T cell receptors and B cell receptors.

Single cell ATAC sequencing

Single cell ATAC sequencing is a technique that assess genome-wide chromatin accessibility by using a transposase to insert sequencing adapters into regions of open chromatin. This analyzes the release information of DNA in a single nucleus while also analyzing the open chromatin region through tagmentation using Tn5 transposase. This can be used to map regions for binding sites of transcription factors and nucleosome locations. Researchers can predict transcription factors with cell-specific binding sites and cell-specific activity when analyzed in a single cell.

Sequencing platform and product

Sequencing platform	NovaSeq 6000 sequencing system, Illumina
Library prep	Chromium Single cell solution, 10x Genomics

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*Research services are currently limited to Korea only.

Research Services

GENOME INSIGHTA precision medicine whole genome platform company.

Research Services 목록

Whole Genome Sequencing

Next generation sequencing service

Transcriptome sequencing

Single cell, multi-omics sequencing

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GENOME INSIGHT
A precision medicine whole genome platform company.